СOLLECTION OF MICROALGAE STRAINS IN THE INSTITUTE OF BIOLOGY OF KOMI SCIENTIFIC CENTRE (SYKOA)

Russian

* Composition of the micronutrient solution (from Kuhl and Lorenzen 1964)

Add to 1000 ml of de-ionized or distilled water:

Make up to 1 litre with distilled water.

Trace element solution
Add to 1000 ml of distilled water 0.75 g Na₂EDTA and the minerals in exactly the following sequence:

WC Medium

Component Stock Solution Quantity Used Concentration in Final

(g · L-1 dH2O) Medium (M)

Buffer (use one, not both)

Glycylglycine — 500 mg 3.78 Ґ 10-3

Tris — 500 mg 4.13 Ґ 10-3

NaNO3 85.01 1 mL 1.00 Ґ 10-3

CaCl2 · 2H2O 36.76 1 mL 2.50 Ґ 10-4

MgSO4 · 7H2O 36.97 1 mL 1.50 Ґ 10-4

NaHCO3 12.60 1 mL 1.50 Ґ 10-4

Na2SiO3 · 9H2O 28.42 1 mL 1.00 Ґ 10-4

K2HPO4 8.71 1 mL 5.00 Ґ 10-5

Trace metals solution (See following recipe) 1 mL —

Vitamins solution (See following recipe) 1 mL —

Appendix A – Recipes for Freshwater and Seawater Media 475

Trace Metals Solution

Into 950 mL of dH2O, add components and bring final volume to 1 liter with dH2O. Autoclave.

Component 1° Stock Solution Quantity Used Concentration in Final

(g · L-1 dH2O) Medium (M)

Na2EDTA · 2H2O — 4.36 g 1.17 Ґ 10-5

FeCl3 · 6H2O — 3.15 g 1.17 Ґ 10-5

CuSO4 · 5H2O 10.0 1 mL 4.01 Ґ 10-8

ZnSO4 · 7H2O 22.0 1 mL 7.65 Ґ 10-8

CoCl2 · 6H2O 10.0 1 mL 4.20 Ґ 10-8

MnCl2 · 4H2O 180.0 1 mL 9.10 Ґ 10-7

Na2MoO4 · 2H2O 6.0 1 mL 2.48 Ґ 10-8

H3BO3 — 1.00 g 1.62 Ґ 10-5

Vitamins Solution

Into 950 mL of dH2O, dissolve the thiamine · HCl, add 1 mL of the primary stocks, and bring final volume to 1

liter with dH2O. Filter-sterilize and store in refrigerator or freezer.

Component 1° Stock Solution Quantity Used Concentration in Final

(g · L-1 dH2O) Medium (M)

Thiamine · HCl (vitamin B1) — 100 mg 2.96 Ґ 10-7

Biotin (vitamin H) 0.5 1 mL 2.05 Ґ 10-9

Cyanocobalamin (vitamin B12) 0.5 1 mL 3.69 Ґ 10-